): punctate and perinuclear. However, to induce cell death, the internalized saporin must gain access to the ribosomes in the cytoplasm. The cell viability results suggest that at least some saporin reaches the cytoplasm. This is likely due to endosomal escape mediated by saporin before trafficking to lysosomes. To observe endosomal escape, a time-course was performed to assess saporin colocalization with lysosomes.
Fig. 5: MGS4_V8 homes to xenograft tumor after systemic delivery and can deliver saporin resulting in reduced tumor growth.H2009 tumor bearing nude mice were injected I.V. with MGS4_V8 or MGS4_V6 conjugated to NIR dye Alexa Fluor 750. At 12, 24, 48, and 72 h postinjection, mice were anesthetized and imaged on an IVIS® to measure total radiant efficiency in each tumor. MGS4_V8 accumulates in tumor 25–39 fold better than control peptide, MGS4_V6.
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