By Pooja Toshniwal PahariaJan 16 2023Reviewed by Danielle Ellis, B.Sc. In a recent study posted to the medRxiv* preprint server, researchers identified a wide variety of pathogenic organisms using plasma microbial cell-free deoxyribonucleic acid sequencing.
However, the usage of the analysis is reportedly limited in regular clinical practices, and data on the diagnostic performance of sequencing mcfDNA from serum for diagnosing infections at a large scale in relation to positivity rates, quality metrics, taxological diversity, and reporting times are scarce.
Documented pathogenic organisms and metrics for laboratory diagnostic performance were evaluated. The team performed serological mcfDNA sequencing at the Karius laboratory. DNA was extracted from the samples, and sequence libraries were prepared.
Fifty-eight percent of reports, belonging to 8,849 individuals, yielded ≥1.0 taxon for 22,792 identifications, covering 701 distinct microbial taxa [bacteria , fungi , viruses , and parasites ]. The 50 most frequent taxa comprised environmental and commensals, including 36 bacterial organisms, nine viral organisms, and five fungal organisms.
In total, 11,023 bacteria were detected, including anaerobes , Streptococcus , Enterobacterales , Staphylococcus , Rothia , Haemophilus , Enterococcus , Acinetobacter haemolyticus , Pseudomonas aeruginosa , Helicobacter pylori , and Stenotrophomonas maltophilia .
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