The Gossypium herbaceum L. Wagad genome as a resource for understanding cotton domestication

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Computerscientists sequence cotton genome DePaulU GeneticsGSA

Plant material and sequencing methodscv. Wagad tissue was collected from a USDA, College Station, TX, greenhouse and shipped to the DNA Sequencing Center at Brigham Young University . DNA was extracted from the plant material using the Qiagen Genomic Tip kit , and sequencing libraries were subsequently constructed and sequenced at the BYU DNA Sequencing Center . DNA shearing of both libraries was done on a Megaruptor2 .

Three PacBio cells were sequenced from 1 circular consensus sequence library on the Pacific Biosciences Sequel system.Plant tissue of Wagad was grown at Brigham Young University Greenhouse. Young tissues were collected, and DNA was extracted through the CTAB method .

 

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