scChIX-seq infers dynamic relationships between histone modifications in single cells - Nature Biotechnology

recombinant murine MCSF . Medium was refreshed after 3 days. Every 24 h, suspension cells were collected and adherent cells were harvested by incubating 10 min in 2 mM EDTA/0.5% BSA in PBS. Suspension and adherent cells were combined and stained with CellTrace fluorescent labels , according to manufacturer’s instructions. Briefly, cells were pelleted and resuspended in 37 °C PBS containing fluorescent dyes : 2.5 μM; CellTrace Yellow : 2.5 μM; CellTrace Far Red : 0.

$$\begin{array}{l}\begin{array}{ll}{{\mbox{antibody}}}\,&\,{{\mbox{info}}}\\ {{\mbox{GR1}}}\,&\,{{\mbox{A647, anti-mouse Ly-6G/Ly-6C Antibody,}}}\\ & {\mbox{clone: RB6-8C5}}\\ {{\mbox{NK1}}}\,&\,{{\mbox{A488, anti-mouse NK-1.

Blunt fragments were subsequently A-tailed by adding 150 nl per well of A-tailing mix and incubated for 15 min at 72 °C. Through the strong preference of AmpliTaq 360 to incorporate dATP as a single base overhang even in the presence of other nucleotides, a general dNTP removal was not necessary. A-tailing mix per well: AmpliTaq 360 1 nl, dATPs 100 mM 1 nl, KCl 1 M 25 nl, PEG8000 50% 7.5 nl, BSA 20 ng 0.8 nl, nuclease-free water 114.8 nl.

oncodeinstitute _knaw UMCUtrecht ISTAustria jakeyeung AlexandervanOu1 Co-lead author jakeyeung goes behindthepaper on quantifying the relationships between histone modifications and how those relationships change across cell types or during differentiation

oncodeinstitute _knaw UMCUtrecht ISTAustria jakeyeung AlexandervanOu1 New methods for research into chromatin NBTintheNews via _Hubrecht

oncodeinstitute _knaw UMCUtrecht ISTAustria jakeyeung AlexandervanOu1 Awesome

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