lymphocytes, as defined by the forward and side scatter gates. Absolute CAR T cell numbers were calculated by multiplying the percentages of CAR T cells among lymphocytes by the absolute lymphocyte count obtained on the same day. The assay limit of detection was calculated as 1 in 10DNA was extracted from 2–5 × 10PBMCs using QIAmp DNA Blood Mini Kit .
. In brief, cryopreserved PBMCs were thawed into 9 ml of cell culture medium supplemented with 25 U ml, resuspended in 10 ml of CCM, filtered through a 70-µm strainer and counted., cisplatin was reconstituted to 100 mM in DMSO and incubated at 37 °C for 3 days to prepare a stock solution, which was then stored in aliquots at −20 °C. Cell pellets were resuspended in 1 ml of PBS containing 0.
Thank you for highlighting our work! We found that this CAR Treg population is Helios+ FOXP3+, and quantification based on either marker led to nearly identical results.
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