Sequences of all oligonucleotides used in this study are available at Additional file 6: Table S5. 6mA modified and unmodified control oligos were synthesized at GeneLink, Inc. All other oligos, including 5mC modified oligo , were synthesized at Sigma. 4mC modified oligo was generated by treating dsDNA oligo with BamHI methyltransferase according to the manufacturer’s instructions .
Microbial community standard DNA was purchased from Zymo Research . One microgram of genomic DNA was first fragmentized to 100–300 bp using Covaris S220 Focused-ultrasonicator. Fragmented gDNA was ligated to TruSeq adaptor using NEBNext Ultra™ II DNA Library Prep Kit for Illumina . Unmodified control DNA was made by amplifying adaptor-ligated DNA using Q5 DNA polymerase . Both native and amplified DNA was first annealed to excessive protective adaptor sequences in 5 mM adaptor oligos and 0.
bcmhouston Nature Interesting. I was just reading about the use of fluorescent dyes to make reagents for cell biology, neuroscience, pharmacology, & imaging. I will save my correlations for my 🧠 for now. elonmusk please save us from Our sin. I’m only 1 Woman with 1 follower & a 1%’er. 🙏💋
bcmhouston Nature . Petrochemicals and agrochemicals are key factors disrupting prenatal and subsequent DNA methylation. The extent of the harmful effects can be measured, sources of pollution identified, and health and aged care costs allocated to producers of harmful chemicals. .
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