were kindly provided by George F. Gao from the Center for Molecular Virology, CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, and Xavier Saelens from the VIB-UGent Center for Medical Biotechnology. Engineered plasmids containing cleavable PTD within the open reading frame were obtained from the WT plasmids via mutagenesis and confirmed by gene sequencing .
Generation of WT influenza viruses and PROTAC influenza viruses harboring cleavable PTD in their genomecells per well from the HEK293T-TEVp cell line under passage 15 and 5 × 10cells per well from the MDCK-TEVp cell line under passage 15 were seeded into six-well plates in DMEM supplemented with 10% FBS and 2 µg mlof puromycin . The co-culture of HEK293T and MDCK.
A new route to vaccines using PROTACs An influenza vaccine is created by attenuating the live virus through targeted proteolysis NBTNV
wyssinstitute SCUCN harvardmed pfizer jnanatx Researchers propose a new live-attenuated influenza A vaccine approach NBTintheNews
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