Epitope binning-seq: A game-changer in antibody drug discovery

Using a fluorescently labeled reference antibody , this method employs flow cytometry to identify similar epitope-binding query antibodies . Next-generation sequencing of fluorescence-negative cells then groups qAbs with epitopes akin to the rAb into epitope bins. This innovative technique holds immense potential in enhancing antibody drug discovery process.

In this study, query antibodies displayed on the surface of cells expressing HER2 were employed. By introducing fluorescently labeled reference antibodies and using flow cytometry analysis, researchers could distinguish between cells where the qAbs masked the epitopes and cells where they did not. Some qAbs effectively blocked the binding of rAb to the antigen, resulting in a population of cells with no rAb binding, termed the rAb-negative population.

The benefits of this platform are significant. Epitope Binning-seq streamlines epitope comparison, enabling rapid identification of antibodies with similar binding patterns, potentially advancing targeted and effective antibody-based therapies. The method's large-scale evaluation can transform antibody characterization, making it possible to assess millions of qAbs simultaneously.

The blood-brain barrier blocks the entry of antibodies into the brain. This limits the potential use of antibody therapeutics to treat brain diseases, such as brain tumors. Elsewhere in the body, ...

Pancreatic Cancer Pharmacology Epigenetics Detectors Biochemistry Organic Chemistry Petroleum

United States Latest News, United States Headlines

Similar News:You can also read news stories similar to this one that we have collected from other news sources.

Scientists develop novel antibody treatment for kidney cancerScientists have built upon BTE technology to develop new and improved recombinant and synthetic DNA versions of therapeutic antibodies that target CA9, called Persistent Multivalent T Cell Engager (CA9-PMTE), that shows promise in pre-clinical models as a potent, long-lasting treatment against ccRCC.
Source: ScienceDaily - 🏆 452. / 53 Read more »

A Monoclonal Antibody Could Help Fight MalariaData suggested that the administration of a single dose of a monoclonal antibody to school-aged children could reduce morbidity within this population.
Source: Medscape - 🏆 386. / 55 Read more »

Researchers take step toward development of universal COVID-19 antibodiesA new human monoclonal antibody moves a step closer to a universal antibody cocktail that works against all strains of SARS-CoV-2.
Source: ScienceDaily - 🏆 452. / 53 Read more »

Multiple Sclerosis Med Nearly Eliminates Disease Activity on MRIA second-generation anti-CD40L monoclonal antibody suppresses multiple sclerosis (MS) disease activity on MRI to an uncommonly high degree, new trial data suggested.
Source: Medscape - 🏆 386. / 55 Read more »

Scientists create tailored drug for aggressive breast cancerScientists have used breast cancer cells' weakness against themselves by linking a tumor-selective antibody with a cell-killing drug to destroy hard-to-treat tumors.
Source: ScienceDaily - 🏆 452. / 53 Read more »

Maintenance Treatment With Guselkumab for Ulcerative Colitis Meets All Endpoints: QUASARThe monoclonal antibody in development as maintenance therapy for people with moderately to severely active ulcerative colitis was more effective than placebo.
Source: Medscape - 🏆 386. / 55 Read more »