We are providing an unedited version of this manuscript to give early access to its findings. Before final publication, the manuscript will undergo further editing. Please note there may be errors present which affect the content, and all legal disclaimers apply.The ability to programme new modes of catalysis into proteins would allow the development of enzyme families with functions beyond those found in nature.
Installation of a genetically encoded photosensitiser into the beta-propeller scaffold of DA_20_00Diels-Alderase into a photoenzyme for [2+2]-cycloadditions . Subsequent development and implementation of a platform for photoenzyme evolution afforded an efficient and enantioselective enzyme that can promote intramolecular and bimolecular cycloadditions, including transformations that have proven challenging to achieve selectively with small molecule catalysts. EnT1.
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