A better view with new mid-infrared nanoscopy

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Biology News

Microbiology,Bacteria,Extreme Survival

A team has constructed an improved mid-infrared microscope, enabling them to see the structures inside living bacteria at the nanometer scale. Mid-infrared microscopy is typically limited by its low resolution, especially when compared to other microscopy techniques.

This latest development produced images at 120 nanometers, which the researchers say is a thirtyfold improvement on the resolution of typical mid-infrared microscopes. Being able to view samples more clearly at this smaller scale can aid multiple fields of research, including into infectious diseases, and opens the way for developing even more accurate mid-infrared-based imaging in the future.

The microscopic realm is where viruses, proteins and molecules dwell. Thanks to modern microscopes, we can venture down to see the inner workings of our very own cells. But even these impressive tools have limitations. For example, super-resolution fluorescent microscopes require specimens to be labeled with fluorescence. This can sometimes be toxic to samples and extended light exposure while viewing can bleach samples, meaning they are no longer useful.

However, in a new breakthrough, researchers at the University of Tokyo have attained a higher resolution of mid-infrared microscopy than ever before."We achieved a spatial resolution of 120 nanometers, that is, 0.12 microns. This amazing resolution is roughly 30 times better than that of conventional mid-infrared microscopy," explained Professor Takuro Ideguchi from the Institute for Photon Science and Technology at the University of Tokyo.

"We were surprised at how clearly we could observe the intracellular structures of bacteria. The high spatial resolution of our microscope could allow us to study, for example, antimicrobial resistance, which is a worldwide issue," said Ideguchi."We believe we can continue to improve the technique in various directions. If we use a better lens and a shorter wavelength of visible light, the spatial resolution could even be below 100 nanometers.

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