3′UTR sequence was amplified from the plasmids produced in the 3′ rapid amplification of cDNA ends of wild-type iPSC-derived motor neurons. The mutation was introduced using the PrimeSTAR Mutagenesis Basal kit , according to the manufacturer's instructions.
The immunofluorescent signal intensity was measured by ZEN software . The quantification of spine number was performed as described previously . Regions of dendrites located 30–100 µm from the cell soma that did not intersect with other dendrites were selected. The numbers of spines, labeled with Alexa Fluor 488-conjugated phalloidin, were counted over a region of 20 µm in length. The number of spines was counted independently, without sample information.
Source: Car News Wire (carnewswire.net)
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