At predetermined time point, organoids were washed twice with PBS and fixed at room temperature for 5 h with 4% PFA , washed with PBS, and placed in 70% ethanol for no more than four days before paraffin embedding. 5 µm sections were stained with Hematoxylin and Eosin . For immunofluorescence staining, first, the slides were deparaffinized and incubated for 1-h with citrate pH 6.0 for antigen retrieval and permeablized with 0.1% v/v Triton-X in PBS for 5 min.
Cells were run through a 40 µm strainer to remove any larger organoid pieces that did not fully digest. Cells were spun at 500 g for 5 min and suspended in cold PBS with 10% FBS. Cells were then spun again, and all groups being stained were suspended in flow staining buffer containing 0.2–4 µg/mL conjugated primary antibody or isotype control . Cells were incubated at room temperature for 45 min in the dark. Cells were spun again and washed 3 times with cold PBS with 10% FBS.
United Kingdom Latest News, United Kingdom Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Source: NewsMedical - 🏆 19. / 71 Read more »
Source: MetroUK - 🏆 13. / 82 Read more »
Source: Glasgow_Times - 🏆 76. / 59 Read more »
Source: Daily_Record - 🏆 9. / 89 Read more »
AstraZeneca raises forecasts after strong Covid and cancer drug sales\n\t\t\tKeep abreast of significant corporate, financial and political developments around the world.\n\t\t\tStay informed and spot emerging risks and opportunities with independent global reporting, expert\n\t\t\tcommentary and analysis you can trust.\n\t\t
Source: FT - 🏆 113. / 51 Read more »
Source: BBCLookNorth - 🏆 37. / 67 Read more »