Advances in automated mass photometry

Sponsored Content by Refeyn Ltd.Reviewed by Olivia FrostJun 27 2024 Automated mass photometry combines the unparalleled simplicity and sensitivity of Refeyn’s molecular mass measurement technology with the efficiency and ease of automation.

Figure 1. The principle of mass photometry. The light scattered by a molecule interacting with the measurement interface interferes with light reflected at that interface. The signal intensity scales linearly with mass. Image Credit: Refeyn Ltd. The TwoMP Auto from Refeyn is easy to use thanks to an intuitive single software interface that allows the operator to directly control the whole data acquisition process.

All the benefits of mass photometry are afforded by automated mass photometry; for example, each experiment takes just minutes, uses a minimal amount of sample, and the results can be intuitively interpreted, all with the more consistent and convenient sample manipulation inherent to automated processes.

Mass photometry is a powerful tool that provides high-resolution distributions of molecular mass directly in a solution while maintaining single-molecule sensitivity. These qualities make it ideally suited to detecting a protein’s different oligomeric states, including rare species representing less than 1 % of a sample population.

Protein concentration was held constant throughout this experiment, while the ligand diadenosine tetraphosphate concentration was varied .

Mass Photometry

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