Nature research paper: Blocking PD-L1–PD-1 improves senescence surveillance and ageing phenotypes
T cells analyzed by FACS from wild type mice fed with normal diet or L-amino acid diet with 60 kcal % fat, 0.1 % methionine, and no added choline for 10 weeks combined with αPD-1 or ABT263 treatments as in Fig., The serum levels of AST, ALT, and LDH from the mice as in. The representative images were shown in upper panels. The percentage of red area in each filed was quantified and described as the hepatic fibrogenesis. Scale bar: 100 μm .
Data are presented as means ± SEM of three or more independent experiments. Two-way ANOVA with Sidak’s test was performed. **P < 0.01, ****P < 0.0001. The precise p-values were described in Methods., Experimental schedule of the PLX3397 treatment on young and old wild type mice. The treatment of 40 mg/kgBW PLX3397 was administrated by oral-gavage twice per week in a total 3 weeks period. .
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