Nature research papers: R-loop-derived cytoplasmic RNA–DNA hybrids activate an immune response - Nature
MCF10A cells into soluble nuclear and cytoplasmic compartments with Lamin B1 and
as markers, respectively. cytoDRIP blot showing cytoplasmic hybrids in asynchronous and serum-starvedMCF10A cells with DMSO or PlaB treatment . Each sample was treated with RH in vitro to confirm the specificity of the hybrid IP. Left, experimental workflow. Right, blots showing hybrids isolated from the cytoplasm or nucleoplasm of siCtrl or siSETX-treated HeLa cells, with mock or LMB treatment prior to harvest. Left, experimental workflow.
is the loading control. GFP ChIP-qPCR in HeLa cells following knockdown of, showing GFP-XPG binding at hybrid sites. ‘Nuc+ Cyto+’ sites were found in the nucleus and cytoplasm, while ‘Nuc+ Cyto-’ sites were only found in the nucleus. Gene names are shown;are intergenic sites. Shown is the mean ± s.d. from three independent biological replicates ; unpaired two-tailed
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