Decoding the secret language of photosynthesis UCRiverside NatureComms
PEP assembly was analyzed via blue-native polyacrylamide gel electrophoresis using a NativePAGE Sample Prep kit and a NativePAGE Novex Bis-Tris Gel system with immunoblot
. One hundred milligrams of seedlings grown under the indicated conditions was ground in liquid nitrogen and resuspended in 3 volumes of BN lysis buffer . Protein extracts were divided into two tubes, one for BN-PAGE and the other for SDS-PAGE. For BN-PAGE, protein extracts were mixed with BN sample buffer , and Benzonase nuclease) and incubated for 60 min at room temperature to degrade DNA/RNA and solubilize the PEP complex. Samples were mixed with 0.
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