Nature research paper: Whole-genome doubling drives oncogenic loss of chromatin segregation
Cell cycle staining
Cells were collected and washed with PBS . Permeabilization was performed in 0.01% Triton X-100 in PBS for 30–60 min at 4 °C. Following PBS washes, the cells were fixed and stained with FxCycle PI/RNase staining solution overnight at 4 °C in the absence of light. Propidium iodide intensity for cell cycle detection was measured using Guava easyCyte and Galios cytometers and analysed using FlowJo .KaryoMAX colcemid solution for 2 h at 37 °C in a humidified incubator.
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