Study demonstrates intrahost MPXV variation within a single lesion CDCgov EIDjournal helsinkiuni mpox mpxv disease infection
By Pooja Toshniwal PahariaJan 31 2023Reviewed by Danielle Ellis, B.Sc. In a recent study published in Emerging Infectious Diseases, researchers reported on the clinical and molecular characteristics of monkeypox virus infections in Finland.
Four MPX patients, aged between 20 and 40 years, were investigated, all of whom had a history of recent travel to South Europe, were self-declared MSM individuals, and reported having unprotected sex in recent times with unknown individuals. Two of the four patients were seropositive for human immunodeficiency virus . Patient 1 presented with fever, inguinal lymphadenopathy, and synchronous MPX lesions on the penis.
Real-time polymerase chain reaction analysis was performed to detect orthopoxvirus presence, to be confirmed as MPXV by hemagglutinin gene sequencing. Further, phylogenetic analysis of MPXV sequences was performed using the phylogenetic tree inferred from the maximum-likelihood technique. The three mutations in the first patient’s sequence comprised minority variations with 10.0% frequency , 12.0% frequency , and 13.0% frequency .
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