Nature research paper: Spatial multiomics map of trophoblast development in early pregnancy
, CHIR99021 1.5 µM, recombinant human R-spondin-1 80 ng ml, A83-01 500 nM, prostaglandin E2 2.5 µM, Y-27632 5 µM. EVTM1: Advanced DMEM/F12 ,-glutamine 2 mM, 2-mercaptoethanol 0.1 mM, penicillin/streptomycin solution 0.5% , BSA 0.3% , ITS-X supplement 1% , NRG1 100 ng ml-glutamine 2 mM, 2-mercaptoethanol 0.1 mM, penicillin/streptomycin solution 0.5% , BSA 0.3% , ITS-X supplement 1% , A83-01 7.5 µM, Knockout serum replacement 4% .
For the multimodal snRNA-seq and scATAC-seq experiments, cells were loaded according to the manufacturer’s protocol for the Chromium Single Cell Multiome ATAC + Gene Expression v1.0 to attain between 2,000 and 10,000 cells per well. Library preparation was carried out according to the manufacturer’s protocol.
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