Successful pregnancy relies directly on the placenta’s complex, dynamic, gene-regulatory networks. Disruption of this vast collection of intercellular and intracellular programs leads to pregnancy complications and developmental defects.
In the present study, we generated a comprehensive, spatially resolved, multimodal cell census elucidating the molecular architecture of the first trimester human placenta. We utilized paired single-nucleus ATAC sequencing and RNA sequencing , spatial snATAC-seq and RNA-seq, and in situ sequencing and hybridization mapping of transcriptomes at molecular resolution to spatially reconstruct the joint epigenomic and transcriptomic regulatory landscape.
Johain R. Ounadjela, Ke Zhang, Kang Jin, Claire Callahan, Francesca Viggiani, Morgan L. Zvezdov, Mingyang Hui, Mattew Simpson & Jian ShuJohain R. Ounadjela, Koseki J. Kobayashi-Kirschvink, Andrew J. C.
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