Nature research paper: Norovirus MLKL-like protein initiates cell death to induce viral egress
NS3-FL, NS3-N and NS3-C in the presence doxycycline for 10 h. Flag-tagged proteins were detected by immunofluorescence with mitochondrial marker COX IV . Scale bar, 10 μm., Representative microscopy imaging of NS3 subcellular localization in HEK293T cells. C-terminal Flag-tagged NS3-FL, NS3-N and NS3-C transfected HEK293T cells, the distribution of NS3 was detected by immunofluorescence with cellular membrane marker . Scale bar, 5 μm.
Full-length, N- or C-terminal of NS3 fused to a C-terminal Flag tag were stably expressed in BV2 cells under a tetracycline-inducible promoter. Doxycycline was used to induce NS3-FL, NS3-N and NS3-C expression. Cells were first gated off forward and side scatter., Flow cytometric analysis of mitochondrial membrane potential in the absence or presence of doxycycline measured by TMRM fluorescence., Flow cytometric analysis of mitochondrial status in the absence or presence doxycycline.
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