Visium spatial transcriptomics is combined with markers for more than 30 proteins
M.S., P.M. and P.R. are current employees of 10x Genomics, Sweden. D.A.L. has served as a consultant for Abbvie, AstraZeneca and Illumina, and is on the Scientific Advisory Board or equity holder of Mission Bio, Pangea, Alethiomics and C2i Genomics. D.A.L. has also received previous research funding from BMS, 10x Genomics, Ultima Genomics and Illumina unrelated to the current manuscript. The other authors declare no competing interests.
After cDNA amplification, ADT and cDNA libraries can be separated and prepared for sequencing to produce indexed ADT libraries and gene expression cDNA libraries . Poly-dT blocking oligo length and wash temperature titration. Titration of different poly-dT blocking oligo lengths and their ability to hinder binding of dual-tagged antibodies to poly-A surface probes at either 4 °C or 37 °C. Note the binding differences at the different temperatures for dT20.
United States Latest News, United States Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Facile repurposing of peptide–MHC-restricted antibodies for cancer immunotherapy - Nature BiotechnologyA method to identify antibodies against peptide–MHC complexes could enable patient-specific cancer immunotherapeutics.
Read more »
A proteome-wide atlas of drug mechanism of action - Nature BiotechnologyA proteome-wide atlas of the effect of 875 compounds reveals mechanisms of action and off-target effects.
Read more »
Telomere-to-telomere assembly of diploid chromosomes with Verkko - Nature BiotechnologyIntegration of long and ultra-long reads results in improved phased, diploid assemblies.
Read more »
Single-cell mapping of combinatorial target antigens for CAR switches using logic gates - Nature BiotechnologySingle-cell mapping of combinatorial target antigens for CAR switches using logic gates
Read more »
Prediction of prime editing insertion efficiencies using sequence features and DNA repair determinants - Nature BiotechnologyPrime editing efficiency is affected by insertion sequence features, secondary structure and DNA repair context.
Read more »