Engineered live bacteria suppress 𝘗𝘴𝘦𝘶𝘥𝘰𝘮𝘰𝘯𝘢𝘴 𝘢𝘦𝘳𝘶𝘨𝘪𝘯𝘰𝘴𝘢 infection in mouse lung and dissolve endotracheal-tube biofilms
flask for 3 d with 5 ml of Hayflick medium without antibiotics and then the conditioned supernatant was filtered with 0.33-µm sterile syringe filters.strains were grown overnight in Erlenmeyer flasks at 37 °C on shaking at 600 r.p.m., and then diluted to an ODculture was then added in triplicate to sterile 96-well, polystyrene microtiter plates. Cells were incubated statically at 37 °C for 24 h to allow for biofilm formation.
Plates were incubated in a TECAN reader at 37 °C with shaking and the ODThe animals used in all the studies were CD1 mice , purchased from Charles River Laboratories, and specific pathogen free. Group animal size were computed with G*Power software. The number of animals used in each experiment is specified in the figure legends.doses and the CV2 strain safety, were performed either at the Institute of Agrobiotechnology facilities or at the Barcelona Biomedical Research Parc facilities .
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