Na] was high , crystal-like plates of staining reagent were observed, and the phage shape was unclear. Moreover, crystal formation was observed in the SEM image. These images were categorized as image A. Only the heads were observable in image A, which was obtained from approximately 57% of the TEM grid area . From the other 40% of the grid, we obtained images categorized as image B, in which staining reagents coated the T4 phages and parts of the carbon film.
However, instances of staining reagents coating T4 phages and clear phage images from only a few percent of the grid were obtained . In image C, the long tail fibers were clearly observable. The concentration of Preyssler compound in the staining solution was directly proportional to the area where image A was observed and inversely proportional to the areas of images B and C. Moreover, the area ratio of image C reached a maximum when the concentration was 0.3 wt%.
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