Frogs regrow amputated legs after treatment with a chemical cocktail

1/27/2022 1:03:00 PM

Adult frogs can’t usually regrow a lost leg, but they can after treatment with a regenerative cocktail – and the new leg even contains functioning nerves

Biodome, Silk Gel

Adult frogs can’t usually regrow a lost leg, but they can after treatment with a device containing a regenerative cocktail – and the new leg even contains functioning nerves

Adult frogs can’t usually regrow a lost leg, but they can after treatment with a regenerative cocktail – and the new leg even contains functioning nerves

First, they amputated the right hindleg of 115 frogs (Xenopus laevis). The frogs were then placed into one of three groups. Those in the first group wore a BioDome over the wound site that had been loaded with a cocktail of five drugs known to help cells regenerate. Frogs in the second group wore BioDomes without the drug cocktail and the third group received no treatment at all. The frogs that wore a BioDome did so for one day, after which it was removed.

“With the cocktail, we put the big signals in to restart the major regenerative pathways,” says Murugan.The researchers tracked limb growth for 18 months. By the end, frogs that received a BioDome containing the drug cocktail regrew legs with digit-like structures at the end. Each frog could use its new leg to stand on, swim and push off walls.

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This just reads creepy... STEGGIEZAURUS Relax frogs, the regenerative cocktail contains just a pinch of MSG (maybe some butter & spices). It isn't as though we intend to harvest, regrow, and again hack off your legs endlessly for use in fine French restaurants.🐸 How bad did that hurt? Is this equivalent to lizards/iguanas growing their tails back? -or better because of the regenerative nerves

That's good to know. Now we just need to convince every frog to carry the equivalent of an epi-pen, just in case, eh? excellent news for frogs’ legs farming industry for cuisse de grenouilles, watch them train. Who writes this stuff? Without NERVES, muscles, bones, tissue and all else in legs it's not a leg.

Is it a squashed frog? we wanna go for a long walk after spinal cord complete injury we want promising successful treatment available for us give us treatment or death instead of life God grant us

Leglike Limb Regrows on Legless Frogs in New ExperimentScientists describe a process by which African clawed frogs can regrow an imperfect but functional lost limb.

Frogs Without Legs Regrow Leglike Limbs in New ExperimentAdult African clawed frogs lose the regeneration skills they had as tadpoles. But scientists have created a process that helps grown frogs regrow missing limbs. Looks pretty perfect to me. Imperfect but functional

Frogs can regrow amputated limbs after being treated with mix of drugsResearchers in the United States said they were able to trigger the regrowth of an amputated leg in a type of African clawed frog. Can they do it on human? Very soon all what we know about adaptation will be modified I'm using Black Crypto Cloud Mining application and earning $100 daily. Download the app now and earn free bitcoins .You have opportunity to earn profit . Initially you can get $20 and then get soon more

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Could Humans Ever Regrow Limbs? A Lab Study With Frogs Offers Hope“They weren’t perfect cosmetically, but they were pretty darn good legs,” a researcher said of the frogs. Yeah because that worked great for Dr. Curtis Connors didn't it ¿

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The researchers have now explored the device’s potential to help in limb regeneration. First, they amputated the right hindleg of 115 frogs ( Xenopus laevis ).m. The frogs were then placed into one of three groups. But these powers dim with maturity. Those in the first group wore a BioDome over the wound site that had been loaded with a cocktail of five drugs known to help cells regenerate. But these powers dim with maturity. Frogs in the second group wore BioDomes without the drug cocktail and the third group received no treatment at all." The technique used by the team of scientists, based at Harvard University's Wyss Institute and Tufts University, involved applying a mix of five drugs to the test frogs' spike-like stump, sealed in with a small silicone dome.

The frogs that wore a BioDome did so for one day, after which it was removed. Now, a group of scientists have found a way to harness the adult frog’s own cells to regrow an imperfect but functional limb. The researchers placed a silicone cap laden with a mixture of regenerative drugs onto an amputation wound for 24 hours. “With the cocktail, we put the big signals in to restart the major regenerative pathways,” says Murugan. The researchers tracked limb growth for 18 months. Over the next 18 months, the frogs gradually regrew what was lost, forming a new leglike structure with nerves, muscles, bones and even toelike projections. By the end, frogs that received a BioDome containing the drug cocktail regrew legs with digit-like structures at the end. The process could guide future research on limb regeneration in humans, but it will be challenging to replicate the results in mammals. Each frog could use its new leg to stand on, swim and push off walls. The process could guide future research on limb regeneration in humans, but it will be challenging to replicate the results in mammals. It could help track endangered animals The research was published in the journal Science Advances on Wednesday.

Read more: Sea creature uses stem cells to regrow entire body from a tiny piece Further investigation showed that the new legs carried nerves, blood vessels and bone in patterns similar to those seen in the original legs. By using a tiny bristle to prod the tips of the limbs and watching how each frog responded, the team found that a similar force was needed to trigger a reaction in the regrown limbs as in original limbs. “I didn’t think we would get the patterning that we did.” “It’s not a full limb that’s regrown,” said Kelly Tseng, a biologist studying regeneration at the University of Nevada, Las Vegas, who was not involved with the research. This confirmed that the nerves in the regrown limbs were functioning. The frogs in the other two groups formed a slender, non-structured flap of tissue called a “spike” at the amputation site. “But it’s certainly a robust response. The frogs that wore a BioDome that lacked the drug cocktail grew slightly longer spikes than the untreated frogs. Some aquatic animals, such as the salamander, have impressive regeneration skills, allowing them to regrow tissue, parts of major organs and even whole limbs."This study is significant because it shows that patterning, albeit not perfect, can be induced in a limb that typically regenerates only a spike," Monaghan said via email.

Frogs that received the BioDome without drugs showed a wide range of sensitivity to prodding of their spikes, while untreated frogs showed a complete lack of feeling in their spikes. Some aquatic animals, such as the salamander, have impressive regeneration skills, allowing them to regrow tissue, parts of major organs and even whole limbs. Levin says the work is exciting and represents the first time that this combination of drugs has been used to trigger limb regeneration. “None of us can say what it feels like to be a frog [with a regrown limb], but from what we can measure, there were no differences compared to a non-injured limb,” says Levin. “They face some of the same limitations as humans do,” said Michael Levin, a biologist who directs the Allen Discovery Center at Tufts University and is an author on the paper. Dr. Although the toes were shorter than normal, the limbs were still growing at the end of the experiment and it is possible they could have eventually formed completely, says Levin. “It is striking that the brief, 24 hours, treatment reported in this work has such a long-lasting effect,” says Lin Gufa at Tongji University in China. Murugan conducted the research in Dr. The strategy the team used relied on triggering dormant mechanisms in the frog's body rather than try to"micromanage its growth," said study author Mike Levin, Vannevar Bush Professor of Biology and director of the Allen Discovery Center at Tufts.

It is simpler and more straightforward compared with methods currently used, such as cell transplantation or multiple rounds of electric stimulation, says Lin. Image An African clawed frog tadpole, which is capable of regenerating tails and limbs until it reaches adulthood. Murugan, Levin and their colleagues also point out in their research paper that these current methods are mostly applied to animals – like axolotls – with a natural ability to regenerate limbs, and not to animals like adult frogs that lack this ability. Image An African clawed frog tadpole, which is capable of regenerating tails and limbs until it reaches adulthood. The researchers are now testing the approach in mammals. Murugan thinks it may have the potential to be tested in humans one day... Journal reference: . "For me, the goal is to identify triggers, very simple kinds of stimuli, that will kick-start the cells and convince them to build whatever it is that you want them to build.