Detection of early seeding of Richter transformation in chronic lymphocytic leukemia - Nature Medicine

  • 📰 NatureMedicine
  • ⏱ Reading Time:
  • 22 sec. here
  • 2 min. at publisher
  • 📊 Qulity Score:
  • News: 12%
  • Publisher: 53%

Single-cell genomic & transcriptomic analyses of longitudinal samples of patients with RichterSyndrome reveal the presence and dynamics of clones driving transformation from chronic lymphocytic leukemia years before clinical manifestation. FerranNadeu

United States Headlines News

United States Latest News, United States Headlines

with minor modifications. Single cells were sorted into 96-well plates containing the lysis buffer . Reverse transcription was performed using SuperScript II in the presence of 1 μM oligo-dT30VN , 1 μM template-switching oligonucleotides and 1 M betaine . cDNA was amplified using the KAPA Hifi Hotstart ReadyMix and IS PCR primer , with 25 cycles of amplification.

-negative live individual cells were sorted with a BD FACSAria Fusion Flow cytometer in 1× PBS supplemented with 0.05% BSA. After FACS, cells were partitioned into Gel Bead In Emulsions by using the Chromium Controller system aiming at a Target Cell Recovery of 5,000 total cells. Sequencing libraries were prepared using the v.3.1 single-cell 3′ mRNA kit . After GEM-RT cleanup, cDNAs were amplified during 14 cycles.

We have summarized this news so that you can read it quickly. If you are interested in the news, you can read the full text here. Read more:

 /  🏆 451. in US
 

Thank you for your comment. Your comment will be published after being reviewed.
Please try again later.

Similar News:You can also read news stories similar to this one that we have collected from other news sources.

Pixies Share Latest Doggerel Single 'Vault of Heaven'Alternative rock veterans Pixies have shared the latest single and video from their upcoming album 'Doggrel.'
Source: SPIN - 🏆 258. / 63 Read more »

FDA clears the way for single monkeypox vaccine doses to be split into fiveHealth care providers can now administer the Jynneos vaccine under the top layer of the skin, a change that allows them to use a lower dosage per patient.
Source: NBCNewsHealth - 🏆 707. / 51 Read more »

Yeah Yeah Yeahs Go Up in Smoke on New Single, 'Burning'Karen O reflects on a fire that nearly destroyed her East Village apartment in the late 1990s on Yeah Yeah Yeahs' new single, 'Burning'
Source: SPIN - 🏆 258. / 63 Read more »

Minor Google Meltdown Exposes The World's Utter Reliance on a Single Tech CompanyEarlier today, reports began emerging Google was down. Not science, engineering.
Source: ScienceAlert - 🏆 63. / 68 Read more »

Watch a single-engine plane crash-land on 91 Freeway in California | AutoblogThe small plane clipped a Toyota pickup truck before spinning out of control, breaking apart and erupting in flames. Firefighters were able to extinguish the fire and remove the wreckage. Looks serious.. I believe, no one got hurt.
Source: therealautoblog - 🏆 528. / 51 Read more »

Disq Returns with Nostalgic New Single 'If Only'Disq channel '90s nostalgia on 'If Only,' the second glimpse of their forthcoming album, 'Desperately Imagining Someplace Quiet.'
Source: PasteMagazine - 🏆 392. / 55 Read more »