. This digital pipeline is applicable for multi-dimensional protein datasets acquired from single-cell multiplexed imaging technologies including IMC and potentially many others. The presented SpatialVizScore scheme quantified the immune spectra of lung cancer tissues and associated patients’ tumors into three main categories including immune inflamed, immune suppressed, and immune cold along a tumor immunity continuum axis.
The nuclear staining was then performed using an Iridium-conjugated intercalator prepared in Maxpar PBS for 30 min at room temperature in a hydration chamber. Finally, the slides were washed with Maxpar water and left to dry at room temperature for 20 minutes. After the staining process is complete, the stained tissues were stored at 4 °C until imaging time .
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