on E9 and harvested at E16.5. Embryos were frozen in OCT, sectioned , fixed, and incubated with an anti-survivin antibody overnight, followed by incubation with a secondary antibody at room temperature for 1 h. Images were collected using a Nikon C2+/60X/1.4 objective and the angle between the two daughter nuclei and the BM was calculated using the “angle” tool in ImageJ.Embryos were injected with lentiviruses encodingon E9 and harvested at E14.5.
Line scan analysis was performed using Fiji. Lines were drawn with the “freehand line tool” with a width of 5 pixels, along the cortex of the cell or through the cell.]. Briefly, E18.5 embryos were collected and immersed in ice-cold methanol gradient in water, taking 2 min per step and then rehydrated using the reverse procedure. Embryos were immersed in 0.2% toluidine blue solution. Embryos were washed in PBS before image capture.
BMCBiology 🙏💪
We have summarized this news so that you can read it quickly. If you are interested in the news, you can read the full text here. Read more:
United Kingdom Latest News, United Kingdom Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Scotland could bake in early July heatwave as map shows hot air over EuropeThe risk of showers will continue into early next week, with conditions set to drastically change during the late part of the week.
Source: Daily_Record - 🏆 9. / 89 Read more »
Adiposity and grip strength: a Mendelian randomisation study in UK Biobank - BMC MedicineBackground Muscle weakness, which increases in prevalence with age, is a major public health concern. Grip strength is commonly used to identify weakness and an improved understanding of its determinants is required. We aimed to investigate if total and central adiposity are causally associated with grip strength. Methods Up to 470,786 UK Biobank participants, aged 38–73 years, with baseline data on four adiposity indicators (body mass index (BMI), body fat percentage (BF%), waist circumference (WC) and waist-hip-ratio (WHR)) and maximum grip strength were included. We examined sex-specific associations between each adiposity indicator and grip strength. We explored whether associations varied by age, by examining age-stratified associations (| 50 years, 50–59 years, 60–64 years,65 years +). Using Mendelian randomisation (MR), we estimated the strength of the adiposity–grip strength associations using genetic instruments for each adiposity trait as our exposure. Results In males, observed and MR associations were generally consistent: higher BMI and WC were associated with stronger grip; higher BF% and WHR were associated with weaker grip: 1-SD higher BMI was associated with 0.49 kg (95% CI: 0.45 kg, 0.53 kg) stronger grip; 1-SD higher WHR was associated with 0.45 kg (95% CI:0.41 kg, 0.48 kg) weaker grip (covariate adjusted observational analyses). Associations of BMI and WC with grip strength were weaker at older ages: in males aged | 50 years and 65 years + , 1-SD higher BMI was associated with 0.93 kg (95% CI: 0.84 kg, 1.01 kg) and 0.13 kg (95% CI: 0.05 kg, 0.21 kg) stronger grip, respectively. In females, higher BF% was associated with weaker grip and higher WC was associated with stronger grip; other associations were inconsistent. Conclusions Using different methods to triangulate evidence, our findings suggest causal links between adiposity and grip strength. Specifically, higher BF% (in both sexes) and WHR (males only) were associated with weaker grip streng
Source: BioMedCentral - 🏆 22. / 71 Read more »
Storing and analyzing a genome on a blockchain - Genome BiologyThere are major efforts underway to make genome sequencing a routine part of clinical practice. A critical barrier to these is achieving practical solutions for data ownership and integrity. Blockchain provides solutions to these challenges in other realms, such as finance. However, its use in genomics is stymied due to the difficulty in storing large-scale data on-chain, slow transaction speeds, and limitations on querying. To overcome these roadblocks, we developed a private blockchain network to store genomic variants and reference-aligned reads on-chain. It uses nested database indexing with an accompanying tool suite to rapidly access and analyze the data.
Source: medical_xpress - 🏆 101. / 51 Read more »
Harmony Tan sends classy message to Serena Williams after Wimbledon shockHarmony Tan has paid tribute to ‘superstar’ Serena Williams after knocking one of her childhood heroes out of Wimbledon.
Source: MetroUK - 🏆 13. / 82 Read more »
Stylist Live is back for 2022Get Friday 11th to Sunday 13th November into your diary because Stylist Live is back! And you don't need to wait long to get your hands on tickets
Source: StylistMagazine - 🏆 133. / 51 Read more »